Amylase potential of filamentous fungi isolated from sweet potato pulp

Vanessa Kamila Mello Grizafis; Jaime Alberti Gomes; Sônia Alvim Veiga Pileggi; Sandra Regina Masetto Antunes; Nátali Maidl de Souza; Pedro Henrique Weirich Neto

doi:10.18011/bioeng.2023.v17.1126

Authors

Vanessa Kamila Mello Grizafis Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
Jaime Alberti Gomes Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0001-5973-5659
Sônia Alvim Veiga Pileggi Graduate Program in Biology, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
Sandra Regina Masetto Antunes Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil; Laboratory of Fuel and Biofuel Analysis (LACBIO), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
Nátali Maidl de Souza Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0002-3621-6781
Pedro Henrique Weirich Neto Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil; Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0002-9039-102X

DOI:

https://doi.org/10.18011/bioeng.2023.v17.1126

Keywords:

Starch, hydrolysis, ethanol, microorganisms, Ipomoea batatas

Abstract

Sweet potato is a rustic culture, widely adapted and with a high starch content, thus having innumerable aptitudes. For the transformation of sweet potatoes into biofuel, some processes are necessary. The success of ethanol production from sweet potatoes depends on the transformation of starch into fermentable sugars. The most used conversion process is enzymatic hydrolysis, which uses commercially available enzymes. However, this process can be carried out through enzymes secreted by filamentous fungi. In this work, filamentous fungi that naturally colonized sweet potatoes were studied. These were isolated, tested for enzymatic activity, and identified by microculture. Fifty-one fungi from the pulp of sweet potatoes Beauregard and BRS-Amélia were isolated, 27 of them showed the ability to hydrolyze starch. Out of these, six showed an enzyme index ≥ 2.0 and were identified as Aspergillus sp. and Penicillium sp. This fact indicates the potential of these fungi in the production of amylase, an important enzyme for ethanol production.

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References

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