ATIVIDADE COMPARATIVA DE POLIFENÓIS TOTAIS E COMPOSTOS ANTIO-XIDANTES DA UNCARIA TOMENTOSA POTENCIADA COM ÁCIDO CÍTRICO

Cesar Augusto Roque Borda; Camila Katerine Aranda Medina; Raiza Felismino Silveira; Priscilla Ayleen Bustos Mac-Lean; Angela Vacaro de Souza; Fernando Ferrari Putti; Eduardo Festozo Vicente

doi:10.18011/bioeng2021v15n1p69-89

Autores

Cesar Augusto Roque Borda Universidade Estadual Paulista (UNESP) https://orcid.org/0000-0002-9262-0383
Camila Katerine Aranda Medina
Raiza Felismino Silveira Universidade Estadual Paulista (UNESP)
Priscilla Ayleen Bustos Mac-Lean Universidade Estadual Paulista (UNESP), Faculdade de Ciências e Engenharia, Departamento de Engenharia de Biossistemas https://orcid.org/
Angela Vacaro de Souza Universidade Estadual Paulista (UNESP), Faculdade de Ciências e Engenharia, Departamento de Engenharia de Biossistemas
Fernando Ferrari Putti Universidade Estadual Paulista (UNESP), Faculdade de Ciências e Engenharia, Departamento de Engenharia de Biossistemas https://orcid.org/0000-0002-0555-9271
Eduardo Festozo Vicente Universidade Estadual Paulista (UNESP), Faculdade de Ciências e Engenharia, Departamento de Engenharia de Biossistemas

DOI:

https://doi.org/10.18011/bioeng2021v15n1p69-89

Palavras-chave:

ABTS, DPPH, Folin-Ciocalteu, FRAP, Efeito sinérgico

Resumo

Uncaria tomentosa, uma trepadeira notável por conter altas concentrações de alcaloides oxindois e compostos fenólicos, é comumente usada na medicina tradicional como agente anti-inflamatório e antioxidante. Além disso, o ácido cítrico é um aditivo alimentar amplamente utilizado como conservante, devido ao seu baixo custo. Dessa forma, este estudo tem como objetivo avaliar o conteúdo de compostos fenólicos de Uncaria tomentosa e avaliar sua atividade antioxidante quando o ácido cítrico, em diferentes concentrações, é adicionado ao extrato. Para isso, foi estabelecido um gradiente de concentrações de ácido cítrico, e o perfil antioxidante de extratos aquosos das folhas e cascas das plantas foram analisados pelo ensaio de Folin-Ciocalteu; inibição do radical livre de 2,2-difenil-1-picril-hidrazil (DPPH); poder antioxidante redutor férrico (FRAP) e capacidade de eliminação de radicais livres catiônicos do ácido 2,2'-azino-bis (ácido 3-etilbenzotiazolina-6-sulfônico) (ABTS). Os resultados mostraram um efeito sinérgico entre o ácido cítrico e os compostos antioxidantes de Uncaria tomentosa, apresentando significância estatística, no qual a o efeito sinérgico foi mais eficiente na casca do que nas folhas.

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