• Cesar Augusto Roque Borda São Paulo State University (UNESP)
  • Camila Katerine Aranda Medina
  • Raiza Felismino Silveira São Paulo State University (UNESP)
  • Priscilla Ayleen Bustos Mac-Lean São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering
  • Angela Vacaro de Souza São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering
  • Fernando Ferrari Putti São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering
  • Eduardo Festozo Vicente São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering



ABTS, DPPH, Folin-Ciocalteu, FRAP, Synergistic Effect


Uncaria tomentosa, a climbing vine notable for containing high concentrations of oxindole alkaloids and phenolic compounds, is commonly used in traditional medicine as an anti-inflammatory and antioxidant agent. Also, the citric acid is a food additive widely used for conservation, due to its low cost. In this way, this study aims to evaluate the content of phenolic compounds from Uncaria tomentosa and investigate its antioxidant activity when citric acid, at different concentrations, is added to the extract. For this purpose, a gradient of citric acid concentrations was established, and the antioxidant profile from a aqueous extracts of the plant leaves and bark was analyzed by Folin-Ciocalteu essay; inhibition of the free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP), and scavenging capacity of cationic free radicals of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed a synergistic effect between citric acid and antioxidant compounds from Uncaria tomentosa, presenting highly statistical significance, the synergistic effect was more efficient in the bark than in the leaves.


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ABDEL-SALAM, Omar ME, et al. Citric Acid an Antioxidant in Liver. En The Liver. Academic Press, 2018. p. 183-198. doi:10.1016/b978-0-12-803951-9.00016-1

ADARAMOLA, Banji; ONIGBINDE, Adebayo. Influence of extraction technique on the mineral content and antioxidant capacity of edible oil extracted from ginger rhizome. Chem Int, 2017, vol. 3, no 1, p. 1-7.

ANTOLOVICH, Michael, et al. Methods for testing antioxidant activity. Analyst, 2002, vol. 127, no 1, p. 183-198.

BECKER, Eleonora Miquel; NTOUMA, Georgia; SKIBSTED, Leif H. Synergism and antagonism between quercetin and other chain-breaking antioxidants in lipid systems of increasing structural organisation. Food Chemistry, 2007, vol. 103, no 4, p. 1288-1296.

BORS, Milena, et al. Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2, 4-dichlorophenol (2, 4-DCP) and catechol. Food and chemical toxicology, 2011, vol. 49, no 9, p. 2202-2211.

BREWER, M. S. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Comprehensive reviews in food science and food safety, 2011, vol. 10, no 4, p. 221-247.

BURIN, Vívian Maria, et al. Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis labrusca grapes: evaluation of different extraction methods. Microchemical Journal, 2014, vol. 114, p. 155-163.

CALEJA, Cristina, et al. Fortification of yogurts with different antioxidant preservatives: A comparative study between natural and synthetic additives. Food chemistry, 2016, vol. 210, p. 262-268.

CHEN, Liang-Yu; CHENG, Chien-Wei; LIANG, Ji-Yuan. Effect of esterification condensation on the Folin-Ciocalteu method for the quantitative measurement of total phenols. Food chemistry, 2015, vol. 170, p. 10-15.

CHIUMARELLI, Marcela, et al. Cassava starch coating and citric acid to preserve quality parameters of fresh‐cut "Tommy Atkins" mango. Journal of food science, 2010, vol. 75, no 5, p. E297-E304.

CRUZ, C. D.; REGAZZI, A. J.; CARNEIRO, P. C. S. Modelos biométricos aplicados ao melhoramento genético (volume 1. Viçosa, Editora UFV, 2004, vol. 1, p. 480p.

DE GUZMAN, Rhet, et al. Synergistic effects of antioxidants on the oxidative stability of soybean oil-and poultry fat-based biodiesel. Journal of the American Oil Chemists' Society, 2009, vol. 86, no 5, p. 459.

DORMAN, H. J. D.; HILTUNEN, Raimo. Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food chemistry, 2004, vol. 88, no 2, p. 193-199.

ERHAN, Sevim Z.; SHARMA, Brajendra K.; PEREZ, Joseph M. Oxidation and low temperature stability of vegetable oil-based lubricants. Industrial Crops and Products, 2006, vol. 24, no 3, p. 292-299.

FARAHANI, Maryam, et al. Chemical characterization and antioxidant activities of Morus alba var. nigra fruits. Scientia Horticulturae, 2019, vol. 253, p. 120-127.

GRIMALT, M., et al. Physicochemical composition and antioxidant activity of three Spanish caper (Capparis spinosa L.) fruit cultivars in three stages of development. Scientia Horticulturae, 2018, vol. 240, p. 509-515.

GRANATO, Daniel, et al. Chemical perspective and criticism on selected analytical methods used to estimate the total content of phenolic compounds in food matrices. TrAC Trends in Analytical Chemistry, 2016, vol. 80, p. 266-279.

KUSKOSKI, E. M., et al. Application of several chemical methods to determine antioxidant activity in fruit pulps. Ciencia y Tecnología de Alimentos, Campinas, 2005, vol. 25, no 4, p. 726-732.

MARINOVA, E.; TONEVA, A.; YANISHLIEVA, N. J. F. C. Synergistic antioxidant effect of α-tocopherol and myricetin on the autoxidation of triacylglycerols of sunflower oil. Food chemistry, 2008, vol. 106, no 2, p. 628-633.

MOLYNEUX, Philip, et al. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. sci. technol, 2004, vol. 26, no 2, p. 211-219.

MUÑOZ-BERNAL, Óscar A., et al. New approach to the interaction between Folin-Ciocalteu reactive and sugars during the quantification of total phenols. TIP Revista Especializada en Ciencias Químico-Biológicas, 2017, vol. 20, no 2, p. 23-28.

NAVARRO, Mirtha, et al. Polyphenolic composition and antioxidant activity of Uncaria tomentosa commercial bark products. Antioxidants, 2019, vol. 8, no 9, p. 339.

OAK, Min-Ho; EL BEDOUI, Jasser; SCHINI-KERTH, Valérie B. Antiangiogenic properties of natural polyphenols from red wine and green tea. The Journal of nutritional biochemistry, 2005, vol. 16, no 1, p. 1-8.

PÉREZ-BURILLO, S., et al. Relationship between composition and bioactivity of persimmon and kiwifruit. Food Research International, 2018, vol. 105, p. 461-472.

PRIORRL, WuX. SchaichK, Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem, 2005, vol. 53, p. 4290-4302.

RAWAT, Devendra S., et al. The effect of binary antioxidant proportions on antioxidant synergy and oxidation stability of Jatropha and Karanja biodiesels. Energy, 2015, vol. 84, p. 643-655.

ROCCULI, Pietro, et al. Effects of the application of anti-browning substances on the metabolic activity and sugar composition of fresh-cut potatoes. Postharvest Biology and Technology, 2007, vol. 43, no 1, p. 151-157.

SÁNCHEZ-RANGEL, Juan Carlos, et al. The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination. Analytical Methods, 2013, vol. 5, no 21, p. 5990-5999.

SANDOVAL, Manuel, et al. Cat's claw inhibits TNFα production and scavenges free radicals: role in cytoprotection. Free Radical Biology and Medicine, 2000, vol. 29, no 1, p. 71-78.

SANDOVAL, M., et al. FM, Angeles, 685 RA Musah, P. Bobrowski, MJ Miller, Antiinflammatory and antioxidant activities of 686 cat's claw (Uncaria tomentosa and Uncaria guianensis) are independent of their 687 alkaloid content. Phytomedicine, 2002, vol. 9, p. 325-337.

SANTOS, Monique Barreto; DA COSTA, Naiara Rocha; GARCIA‐ROJAS, Edwin Elard. Interpolymeric complexes formed between whey proteins and biopolymers: Delivery systems of bioactive ingredients. Comprehensive Reviews in Food Science and Food Safety, 2018, vol. 17, no 3, p. 792-805.

SHARMA, B. K.; PEREZ, J. M. k Erhan, SZ 20076 Soybean oil-based lubricants: a search for synergistic antioxidants. Energy Fuels, vol. 21, p. 2408-2414.

DE SOUZA, Angela Vacaro; DA SILVA VIEIRA, Marcos Ribeiro; PUTTI, Fernando Ferrari. Correlations between the phenolic compounds and antioxidant activity in the skin and pulp of table grape varieties. Brazilian Journal of Food Technology, 2018.

SUJA, Kizhiyedathu Polachira; JAYALEKSHMY, Anathasankaran; ARUMUGHAN, Chami. Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH• system. Journal of agricultural and food chemistry, 2004, vol. 52, no 4, p. 912-915.

TAGLIAZUCCHI, Davide, et al. In vitro bio-accessibility and antioxidant activity of grape polyphenols. Food Chemistry, 2010, vol. 120, no 2, p. 599-606.

TANG, Haiying, et al. Effect of antioxidants on the storage stability of soybean-oil-based biodiesel. Energy & fuels, 2010, vol. 24, no 3, p. 2028-2033.

TANG, L., et al. 2008. Interactions among Ytm1, Erb1 and Nop7 required for assembly of the Nop7-subcomplex in yeast pre-ribosomes. Mol Biol Cell, vol. 19, p. 2844-2856.

VÍTOLO, Hilton F.; SOUZA, Gustavo M.; SILVEIRA, Joaquim AG. Cross-scale multivariate analysis of physiological responses to high temperature in two tropical crops with C3 and C4 metabolism. Environmental and Experimental Botany, 2012, vol. 80, p. 54-62.

ZAOUALI, Yosr; BOUZAINE, Taroub; BOUSSAID, Mohamed. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and chemical toxicology, 2010, vol. 48, no 11, p. 3144-3152.




How to Cite

ROQUE BORDA, C. A.; KATERINE ARANDA MEDINA, C.; FELISMINO SILVEIRA, R.; AYLEEN BUSTOS MAC-LEAN, P.; VACARO DE SOUZA, A.; FERRARI PUTTI, F.; FESTOZO VICENTE, E. COMPARATIVE ACTIVITY OF TOTAL POLYPHENOLS AND ANTIOXIDANT COMPOUNDS FROM UNCARIA TOMENTOSA ENHANCED WITH CITRIC ACID. Revista Brasileira de Engenharia de Biossistemas, Tupã, São Paulo, Brazil, v. 15, n. 1, p. 69–89, 2021. DOI: 10.18011/bioeng2021v15n1p69-89. Disponível em: Acesso em: 25 sep. 2021.