COMPARATIVE ACTIVITY OF TOTAL POLYPHENOLS AND ANTIOXIDANT COMPOUNDS FROM UNCARIA TOMENTOSA ENHANCED WITH CITRIC ACID
Keywords:ABTS, DPPH, Folin-Ciocalteu, FRAP, Synergistic Effect
Uncaria tomentosa, a climbing vine notable for containing high concentrations of oxindole alkaloids and phenolic compounds, is commonly used in traditional medicine as an anti-inflammatory and antioxidant agent. Also, the citric acid is a food additive widely used for conservation, due to its low cost. In this way, this study aims to evaluate the content of phenolic compounds from Uncaria tomentosa and investigate its antioxidant activity when citric acid, at different concentrations, is added to the extract. For this purpose, a gradient of citric acid concentrations was established, and the antioxidant profile from a aqueous extracts of the plant leaves and bark was analyzed by Folin-Ciocalteu essay; inhibition of the free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP), and scavenging capacity of cationic free radicals of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed a synergistic effect between citric acid and antioxidant compounds from Uncaria tomentosa, presenting highly statistical significance, the synergistic effect was more efficient in the bark than in the leaves.
ABDEL-SALAM, Omar ME, et al. Citric Acid an Antioxidant in Liver. En The Liver. Academic Press, 2018. p. 183-198. doi:10.1016/b978-0-12-803951-9.00016-1 https://doi.org/10.1016/B978-0-12-803951-9.00016-1
ADARAMOLA, Banji; ONIGBINDE, Adebayo. Influence of extraction technique on the mineral content and antioxidant capacity of edible oil extracted from ginger rhizome. Chem Int, 2017, vol. 3, no 1, p. 1-7.
ANTOLOVICH, Michael, et al. Methods for testing antioxidant activity. Analyst, 2002, vol. 127, no 1, p. 183-198. https://doi.org/10.1039/b009171p
BECKER, Eleonora Miquel; NTOUMA, Georgia; SKIBSTED, Leif H. Synergism and antagonism between quercetin and other chain-breaking antioxidants in lipid systems of increasing structural organisation. Food Chemistry, 2007, vol. 103, no 4, p. 1288-1296. https://doi.org/10.1016/j.foodchem.2006.10.034
BORS, Milena, et al. Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2, 4-dichlorophenol (2, 4-DCP) and catechol. Food and chemical toxicology, 2011, vol. 49, no 9, p. 2202-2211. https://doi.org/10.1016/j.fct.2011.06.013
BREWER, M. S. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Comprehensive reviews in food science and food safety, 2011, vol. 10, no 4, p. 221-247. https://doi.org/10.1111/j.1541-4337.2011.00156.x
BURIN, Vívian Maria, et al. Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis labrusca grapes: evaluation of different extraction methods. Microchemical Journal, 2014, vol. 114, p. 155-163. https://doi.org/10.1016/j.microc.2013.12.014
CALEJA, Cristina, et al. Fortification of yogurts with different antioxidant preservatives: A comparative study between natural and synthetic additives. Food chemistry, 2016, vol. 210, p. 262-268. https://doi.org/10.1016/j.foodchem.2016.04.114
CHEN, Liang-Yu; CHENG, Chien-Wei; LIANG, Ji-Yuan. Effect of esterification condensation on the Folin-Ciocalteu method for the quantitative measurement of total phenols. Food chemistry, 2015, vol. 170, p. 10-15. https://doi.org/10.1016/j.foodchem.2014.08.038
CHIUMARELLI, Marcela, et al. Cassava starch coating and citric acid to preserve quality parameters of fresh‐cut "Tommy Atkins" mango. Journal of food science, 2010, vol. 75, no 5, p. E297-E304. https://doi.org/10.1111/j.1750-3841.2010.01636.x
CRUZ, C. D.; REGAZZI, A. J.; CARNEIRO, P. C. S. Modelos biométricos aplicados ao melhoramento genético (volume 1. Viçosa, Editora UFV, 2004, vol. 1, p. 480p.
DE GUZMAN, Rhet, et al. Synergistic effects of antioxidants on the oxidative stability of soybean oil-and poultry fat-based biodiesel. Journal of the American Oil Chemists' Society, 2009, vol. 86, no 5, p. 459. https://doi.org/10.1007/s11746-009-1373-8
DORMAN, H. J. D.; HILTUNEN, Raimo. Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food chemistry, 2004, vol. 88, no 2, p. 193-199. https://doi.org/10.1016/j.foodchem.2003.12.039
ERHAN, Sevim Z.; SHARMA, Brajendra K.; PEREZ, Joseph M. Oxidation and low temperature stability of vegetable oil-based lubricants. Industrial Crops and Products, 2006, vol. 24, no 3, p. 292-299. https://doi.org/10.1016/j.indcrop.2006.06.008
FARAHANI, Maryam, et al. Chemical characterization and antioxidant activities of Morus alba var. nigra fruits. Scientia Horticulturae, 2019, vol. 253, p. 120-127. https://doi.org/10.1016/j.scienta.2019.04.040
GRIMALT, M., et al. Physicochemical composition and antioxidant activity of three Spanish caper (Capparis spinosa L.) fruit cultivars in three stages of development. Scientia Horticulturae, 2018, vol. 240, p. 509-515. https://doi.org/10.1016/j.scienta.2018.06.061
GRANATO, Daniel, et al. Chemical perspective and criticism on selected analytical methods used to estimate the total content of phenolic compounds in food matrices. TrAC Trends in Analytical Chemistry, 2016, vol. 80, p. 266-279. https://doi.org/10.1016/j.trac.2016.03.010
KUSKOSKI, E. M., et al. Application of several chemical methods to determine antioxidant activity in fruit pulps. Ciencia y Tecnología de Alimentos, Campinas, 2005, vol. 25, no 4, p. 726-732. https://doi.org/10.1590/S0101-20612005000400016
MARINOVA, E.; TONEVA, A.; YANISHLIEVA, N. J. F. C. Synergistic antioxidant effect of α-tocopherol and myricetin on the autoxidation of triacylglycerols of sunflower oil. Food chemistry, 2008, vol. 106, no 2, p. 628-633. https://doi.org/10.1016/j.foodchem.2007.06.022
MOLYNEUX, Philip, et al. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. sci. technol, 2004, vol. 26, no 2, p. 211-219.
MUÑOZ-BERNAL, Óscar A., et al. New approach to the interaction between Folin-Ciocalteu reactive and sugars during the quantification of total phenols. TIP Revista Especializada en Ciencias Químico-Biológicas, 2017, vol. 20, no 2, p. 23-28.
NAVARRO, Mirtha, et al. Polyphenolic composition and antioxidant activity of Uncaria tomentosa commercial bark products. Antioxidants, 2019, vol. 8, no 9, p. 339. https://doi.org/10.3390/antiox8090339
OAK, Min-Ho; EL BEDOUI, Jasser; SCHINI-KERTH, Valérie B. Antiangiogenic properties of natural polyphenols from red wine and green tea. The Journal of nutritional biochemistry, 2005, vol. 16, no 1, p. 1-8. https://doi.org/10.1016/j.jnutbio.2004.09.004
PÉREZ-BURILLO, S., et al. Relationship between composition and bioactivity of persimmon and kiwifruit. Food Research International, 2018, vol. 105, p. 461-472. https://doi.org/10.1016/j.foodres.2017.11.022
PRIORRL, WuX. SchaichK, Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem, 2005, vol. 53, p. 4290-4302. https://doi.org/10.1021/jf0502698
RAWAT, Devendra S., et al. The effect of binary antioxidant proportions on antioxidant synergy and oxidation stability of Jatropha and Karanja biodiesels. Energy, 2015, vol. 84, p. 643-655. https://doi.org/10.1016/j.energy.2015.03.024
ROCCULI, Pietro, et al. Effects of the application of anti-browning substances on the metabolic activity and sugar composition of fresh-cut potatoes. Postharvest Biology and Technology, 2007, vol. 43, no 1, p. 151-157. https://doi.org/10.1016/j.postharvbio.2006.08.002
SÁNCHEZ-RANGEL, Juan Carlos, et al. The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination. Analytical Methods, 2013, vol. 5, no 21, p. 5990-5999. https://doi.org/10.1039/c3ay41125g
SANDOVAL, Manuel, et al. Cat's claw inhibits TNFα production and scavenges free radicals: role in cytoprotection. Free Radical Biology and Medicine, 2000, vol. 29, no 1, p. 71-78. https://doi.org/10.1016/S0891-5849(00)00327-0
SANDOVAL, M., et al. FM, Angeles, 685 RA Musah, P. Bobrowski, MJ Miller, Antiinflammatory and antioxidant activities of 686 cat's claw (Uncaria tomentosa and Uncaria guianensis) are independent of their 687 alkaloid content. Phytomedicine, 2002, vol. 9, p. 325-337. https://doi.org/10.1078/0944-7113-00117
SANTOS, Monique Barreto; DA COSTA, Naiara Rocha; GARCIA‐ROJAS, Edwin Elard. Interpolymeric complexes formed between whey proteins and biopolymers: Delivery systems of bioactive ingredients. Comprehensive Reviews in Food Science and Food Safety, 2018, vol. 17, no 3, p. 792-805. https://doi.org/10.1111/1541-4337.12350
SHARMA, B. K.; PEREZ, J. M. k Erhan, SZ 20076 Soybean oil-based lubricants: a search for synergistic antioxidants. Energy Fuels, vol. 21, p. 2408-2414. https://doi.org/10.1021/ef0605854
DE SOUZA, Angela Vacaro; DA SILVA VIEIRA, Marcos Ribeiro; PUTTI, Fernando Ferrari. Correlations between the phenolic compounds and antioxidant activity in the skin and pulp of table grape varieties. Brazilian Journal of Food Technology, 2018.
SUJA, Kizhiyedathu Polachira; JAYALEKSHMY, Anathasankaran; ARUMUGHAN, Chami. Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH• system. Journal of agricultural and food chemistry, 2004, vol. 52, no 4, p. 912-915. https://doi.org/10.1021/jf0303621
TAGLIAZUCCHI, Davide, et al. In vitro bio-accessibility and antioxidant activity of grape polyphenols. Food Chemistry, 2010, vol. 120, no 2, p. 599-606. https://doi.org/10.1016/j.foodchem.2009.10.030
TANG, Haiying, et al. Effect of antioxidants on the storage stability of soybean-oil-based biodiesel. Energy & fuels, 2010, vol. 24, no 3, p. 2028-2033. https://doi.org/10.1021/ef9012032
TANG, L., et al. 2008. Interactions among Ytm1, Erb1 and Nop7 required for assembly of the Nop7-subcomplex in yeast pre-ribosomes. Mol Biol Cell, vol. 19, p. 2844-2856. https://doi.org/10.1091/mbc.e07-12-1281
VÍTOLO, Hilton F.; SOUZA, Gustavo M.; SILVEIRA, Joaquim AG. Cross-scale multivariate analysis of physiological responses to high temperature in two tropical crops with C3 and C4 metabolism. Environmental and Experimental Botany, 2012, vol. 80, p. 54-62. https://doi.org/10.1016/j.envexpbot.2012.02.002
ZAOUALI, Yosr; BOUZAINE, Taroub; BOUSSAID, Mohamed. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and chemical toxicology, 2010, vol. 48, no 11, p. 3144-3152. https://doi.org/10.1016/j.fct.2010.08.010
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