ATIVIDADE COMPARATIVA DE POLIFENÓIS TOTAIS E COMPOSTOS ANTIO-XIDANTES DA UNCARIA TOMENTOSA POTENCIADA COM ÁCIDO CÍTRICO

Cesar Augusto Roque Borda; Camila Katerine Aranda Medina; Raiza Felismino Silveira; Priscilla Ayleen Bustos Mac-Lean; Angela Vacaro de Souza; Fernando Ferrari Putti; Eduardo Festozo Vicente

doi:10.18011/bioeng2021v15n1p69-89

Authors

Cesar Augusto Roque Borda São Paulo State University (UNESP) https://orcid.org/0000-0002-9262-0383
Camila Katerine Aranda Medina
Raiza Felismino Silveira São Paulo State University (UNESP)
Priscilla Ayleen Bustos Mac-Lean São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering https://orcid.org/
Angela Vacaro de Souza São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering
Fernando Ferrari Putti São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering https://orcid.org/0000-0002-0555-9271
Eduardo Festozo Vicente São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering

DOI:

https://doi.org/10.18011/bioeng2021v15n1p69-89

Keywords:

ABTS, DPPH, Folin-Ciocalteu, FRAP, Synergistic Effect

Abstract

Uncaria tomentosa, a climbing vine notable for containing high concentrations of oxindole alkaloids and phenolic compounds, is commonly used in traditional medicine as an anti-inflammatory and antioxidant agent. Also, the citric acid is a food additive widely used for conservation, due to its low cost. In this way, this study aims to evaluate the content of phenolic compounds from Uncaria tomentosa and investigate its antioxidant activity when citric acid, at different concentrations, is added to the extract. For this purpose, a gradient of citric acid concentrations was established, and the antioxidant profile from a aqueous extracts of the plant leaves and bark was analyzed by Folin-Ciocalteu essay; inhibition of the free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP), and scavenging capacity of cationic free radicals of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed a synergistic effect between citric acid and antioxidant compounds from Uncaria tomentosa, presenting highly statistical significance, the synergistic effect was more efficient in the bark than in the leaves.

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