COMPARATIVE ACTIVITY OF TOTAL POLYPHENOLS AND ANTIOXIDANT COMPOUNDS FROM UNCARIA TOMENTOSA ENHANCED WITH CITRIC ACID

Authors

  • Cesar Augusto Roque Borda São Paulo State University (UNESP) https://orcid.org/0000-0002-9262-0383
  • Camila Katerine Aranda Medina
  • Raiza Felismino Silveira São Paulo State University (UNESP)
  • Priscilla Ayleen Bustos Mac-Lean São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering https://orcid.org/
  • Angela Vacaro de Souza São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering
  • Fernando Ferrari Putti São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering https://orcid.org/0000-0002-0555-9271
  • Eduardo Festozo Vicente São Paulo State University (UNESP), School of Science and Engineering, Department of Biosystems Engineering

DOI:

https://doi.org/10.18011/bioeng2021v15n1p69-89

Keywords:

ABTS, DPPH, Folin-Ciocalteu, FRAP, Synergistic Effect

Abstract

Uncaria tomentosa, a climbing vine notable for containing high concentrations of oxindole alkaloids and phenolic compounds, is commonly used in traditional medicine as an anti-inflammatory and antioxidant agent. Also, the citric acid is a food additive widely used for conservation, due to its low cost. In this way, this study aims to evaluate the content of phenolic compounds from Uncaria tomentosa and investigate its antioxidant activity when citric acid, at different concentrations, is added to the extract. For this purpose, a gradient of citric acid concentrations was established, and the antioxidant profile from a aqueous extracts of the plant leaves and bark was analyzed by Folin-Ciocalteu essay; inhibition of the free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP), and scavenging capacity of cationic free radicals of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The results showed a synergistic effect between citric acid and antioxidant compounds from Uncaria tomentosa, presenting highly statistical significance, the synergistic effect was more efficient in the bark than in the leaves.

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Published

2021-04-20

How to Cite

ROQUE BORDA, C. A.; KATERINE ARANDA MEDINA, C.; FELISMINO SILVEIRA, R.; AYLEEN BUSTOS MAC-LEAN, P.; VACARO DE SOUZA, A.; FERRARI PUTTI, F.; FESTOZO VICENTE, E. COMPARATIVE ACTIVITY OF TOTAL POLYPHENOLS AND ANTIOXIDANT COMPOUNDS FROM UNCARIA TOMENTOSA ENHANCED WITH CITRIC ACID. Revista Brasileira de Engenharia de Biossistemas, Tupã, São Paulo, Brazil, v. 15, n. 1, p. 69–89, 2021. DOI: 10.18011/bioeng2021v15n1p69-89. Disponível em: https://seer.tupa.unesp.br/index.php/BIOENG/article/view/986. Acesso em: 25 sep. 2021.

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