DEVELOPMENT OF PLANT CLONING TECHNIQUE BY IN VITRO STARTING PROCESS USING VITAMIN B1 AS A ROOTING REGULATOR

Abstract

This work aimed to develop a cloning methodology combining cutting and in vitro reproduction techniques performed in laboratories. The cutting technique uses high concentrations of rooting regulator substances and it is difficult to visualize root development because it is inserted directly into the soil. In vitro technique demands sophisticated laboratory infrastructure and operations. Because of that, both technologies are quite expensive. Thus, the objective was to study a new methodology that did not require sophisticated laboratory infrastructure or elevated costs, and allowed great visual monitoring of the studies and had a good reproduction effectiveness. For reduce input costs, vitamin B1 (Thiamine Hydrochloride) was used as a regulator substance and it was mixed with a solution of macro and micronutrients at concentrations optimized for the plant development. On the culture substrate, was added 0.8% of Agar to form a layer of nutrients in gel where were added stains of the species Callianthe striata (Dicks. ex Lindl.) Donnel (Malvaceae) were conditioned for the accomplishment of the experiments. After 30 days of cloning germination, was observed around 40 to 50% of the stains with the appearance of leaves and roots. The plants with better root and leaf development were transferred to pots with nutrient substrates in order to evaluate the evolution of reproduction. After two years, it was possible to observe healthy plants with similar flowering rates to the matrix. This new cloning technique is deemed low cost, without requiring sophisticated laboratory infrastructure and is easy to operate.