A USEFUL METHODOLOGY TO SELECT LIPASE-CATALYZED TRANSESTERIFICATION AIMING BIODIESEL APPLICATION
DOI:
https://doi.org/10.18011/bioeng2016v10n1p01-13Keywords:
Lipase, Transesterificação, BiodieselAbstract
The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO4.7H2O, 0.015% KH2PO4, 0.05% NH4H2PO4. Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production.
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ABBAS, H.; HIOL, A.; DEYRIS, V.; COMEAU, L. (2002) Isolation and characterization of an extracellular lipase from Mucor sp strain isolated from palm fruit. Enzyme Microb Technol 31(7):968-975.
ACIKEL, U.; ERSAN, M.; ACIKEL, Y.S. (2011) The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turk J Biol 35(1):35-44.
ADAMS, P.R. (1990) Mycelial amylase activities of thermophilic species of Rhizomucor, Humicola and Papulaspora. Mycopathologia 112(1):35-37.
AKBARI, N.; DANESHJOO, S.; AKBARI, J.; KHAJEH, K. (2011) Isolation, characterization, and catalytic properties of a novel lipase which is activated in ionic liquids and organic solvents. Appl Biochem Biotechnol 165(3-4):785-794.
ANDRADE, M.M.; BARBOSA, A.M.; BONFINGER, M.R.; DEKKER, R.F.H.; MESSIAS, J.M.; GUEDES, C.L.B.; ZAMIELLI, T.; OLIVEIRA, B.H.; LIMA, V.M.G.; DALL'ANTONIA, L.H. (2013) Lipase production by Botryosphaeria ribis EC-01 on soybean and castorbean meals: optimization, immobilization, and application for biodiesel production. Appl Biochem Biotechnol 170(7):1792-1806.
BRADFORD, M.M. (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 72(1-2):248-254.
CAMARGO-DE-MORAIS, M.M.; MAIA, M.M.D.; BORBA, F.F.S.; MELO, K.G; SANTOS, C.; REIS, E.R.A.; MORAIS, M.A.; LIMA, J.L. (2003) Oil/mineral-salts medium designed for easy recovery of extracellular lipase from Fusarium oxysporum AM3. World J Microbiol Biotechnol 19(1):17-20.
FACCHINI, F.D.A.; PEREIRA, M.G.; VICI, A.C. Lipases: imperative fat-degrading enzymes. (2013) In: Polizeli M.L.T.M.; Rai, M.K., Editors. Fungal Enzymes. New York: CRC Press. pp.392-42.
GOUJARD, L.; VILLENEUVE, P.; BAREA, B.; LECOMTE, J.; PINA, M.; CLAUDE, S.; LE PETIT, J.; FERRE, E. (2009) A spectrophotometric transesterification-based assay for lipases in organic solvent. Anal Biochem 385(1):161-167.
GUPTA, R.; RATHI, P.; GUPTA, N.; BRADOO, S. (2003) Lipase assays for conventional and molecular screening: an overview. Biotechnol Appl Biochem 37:63-71.
HASAN, F.; SHAH, A.A.; HAMEED, A. (2009) Methods for detection and characterization of lipases: A comprehensive review. Biotechnol Adv 27(6):782-798.
JAEGER, K.E.; EGGERT, T. (2002) Lipases for biotechnology. Curr Opin Biotechnol 13:390-397.
KAUSHIK, R.; MARWAH, R.G.; GUPTA, P.; SARAN, S.; SASO, L.; PARMAR, V.S.; SAXENA, R.K. (2010) Optimization of lipase production from Aspergillus terreus by response surface methodology and its potential for synthesis of partial glycerides under solvent free conditions. Indian J Microbiol 50(4):456-462.
KHANNA, P.; SUNDARI, S.S.; KUMAR, N.J. (1995) Production, isolation and partial-purification of xylanases from an Aspergillus sp. World J Microbiol Biotechnol 11(2):242-243.
LIMA, V.M.G.; KRIEGER, N.; SARQUIS, M.I.M.; MITCHELL, D.A.; RAMOS, L.P.; FONTANA, J.D. (2003) Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum. Food Technol Biotechnol 41(2):105-110.
LIU, J.; ZHANG, Y. (2011). Optimization of lipase production by a mutant of Candida antarctica DSM-3855 using response surface methodology. Int J Food Sci Technol 46(4):695-701.
PENCREACH, G.; BARATTI, J.C. (1996) Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media. Enzyme Microb Technol 18(6):417-422.
PEREIRA, M.G.; VICI, A.C.; FACCHINI, F.D.A.; TRISTÃO, A.P.; CURSINO-SANTOS, J.R.; SANCHES, P.R.; JORGE, J.A.; POLIZELI, M.L.T.M. (2014) Screening of filamentous fungi for lipase production: Hypocrea pseudokonigii a new producer with a high biotechnological potencial. Biocatal Biotransfor 32(1):74-83.
PRAZERES, J.N.; BORTOLLOTTI CRUZ, J.A; PASTORE, G.M. (2006) Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity. Braz J Microbiol 37(4):505-509.
RAPP, P. (1995) Production, regulation, and some properties of lipase activity from Fusarium oxysporum f. sp. vasinfectum. Enzyme Microb Technol 17(9):832-838.
RIZZATTI, A.C.S.; JORGE, J.A.; TERENZI, H.F.; RECHIA, C.G.V.; POLIZELI M.L.T.M. (2001) Purification and properties of a thermostable extracellular beta-D-xylosidase produced by a thermotolerant Aspergillus phoenicis. J Ind Microbiol Biotechnol 26(3):156-160.
SHAH, S.; SHARMA, S.; GUPTA, M.N. (2004) Biodiesel preparation by lipase-catalyzed transesterification of Jatropha oil. Energy Fuels 18(1):154-159.
SHELLEY, A.W.; DEETH, H.C.; MACRAE, I.C. (1987) Review of methods of enumeration, detection and isolation of lipolytic microorganisms with special reference to dairy applications. J Microbiol Meth 6:123-137.
TENG, Y.; XU, Y. (2007) A modified para-nitrophenyl palmitate assay for lipase synthetic activity determination in organic solvent. Anal Biochem 363(2):297-299.
WANG, D.; XU, Y.; SHAN, T. (2008) Effects of oils and oil-related substrates on the synthetic activity of membrane-bound lipase from Rhizopus chinensis and optimization of the lipase fermentation media. Biochem Eng J 41(1), 30-37.
WISEMAN, A. (1975) Editor. Handbook of Enzyme Biotechnology. New York: Ellis Horwood Ltd., John Wiley and Sons. 139p.
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Copyright (c) 2016 F. D. A. Facchini, A. C. Vici, M. G. Pereira, M. F. de Oliveira, A. C. F. Batista, A. T. Vieira, T. A. Silva, J. A. Jorge, M. L. T. M. Polizeli
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