Amylase potential of filamentous fungi isolated from sweet potato pulp

Autores

  • Vanessa Kamila Mello Grizafis Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
  • Jaime Alberti Gomes Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0001-5973-5659
  • Sônia Alvim Veiga Pileggi Graduate Program in Biology, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
  • Sandra Regina Masetto Antunes Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil; Laboratory of Fuel and Biofuel Analysis (LACBIO), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil
  • Nátali Maidl de Souza Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0002-3621-6781
  • Pedro Henrique Weirich Neto Graduate Program in Bioenergy, State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil; Laboratory of Agricultural Mechanization (Lama), State University of Ponta Grossa – UEPG, Ponta Grossa, PR, Brazil https://orcid.org/0000-0002-9039-102X

DOI:

https://doi.org/10.18011/bioeng.2023.v17.1126

Palavras-chave:

Starch, hydrolysis, ethanol, microorganisms, Ipomoea batatas

Resumo

Sweet potato is a rustic culture, widely adapted and with a high starch content, thus having innumerable aptitudes. For the transformation of sweet potatoes into biofuel, some processes are necessary. The success of ethanol production from sweet potatoes depends on the transformation of starch into fermentable sugars. The most used conversion process is enzymatic hydrolysis, which uses commercially available enzymes. However, this process can be carried out through enzymes secreted by filamentous fungi. In this work, filamentous fungi that naturally colonized sweet potatoes were studied. These were isolated, tested for enzymatic activity, and identified by microculture. Fifty-one fungi from the pulp of sweet potatoes Beauregard and BRS-Amélia were isolated, 27 of them showed the ability to hydrolyze starch. Out of these, six showed an enzyme index ≥ 2.0 and were identified as Aspergillus sp. and Penicillium sp. This fact indicates the potential of these fungi in the production of amylase, an important enzyme for ethanol production.

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Referências

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Rodrigues, C.; Cassini, S. T.; Antunes, P. W.; Keller, R. P.; Gonçalves, R. F. (2016). Isolamento e seleção de fungos produtores de lípase com base na atividade lipásica e no potencial hidrolítico sobre óleo comestível de soja e escuma de caixa de gordura. Revista Engenharia Sanitária e Ambiental, v. 21, n. 3, p. 507-518. https://doi.org/10.1590/S1413-41522016141401 DOI: https://doi.org/10.1590/S1413-41522016141401

Sakai, P.; Afionis, S.; Favretto, N.; Stringer, L. C.; Ward, C.; Sakai, M.; Weirich Neto, P. H.; Rocha, C. H.; Gomes, J. A.; Souza, N. M.; Afzal, N. (2020). Understanding the implications of alternative bioenergy crops to support smallholder farmers in Brazil. Sustainability, v. 12, 2146. https://doi.org/10.3390/su12052146 DOI: https://doi.org/10.3390/su12052146

Salla, D. A.; Furlaneto, F. P. B.; Cabello, C.; Kanthack, R. A. D. (2010). Análise energética de sistemas de produção de etanol de mandioca (Manihot esculenta Crantz. R. Bras. Eng. Agríc. Ambiental, v.14, n.4, p.444–448. DOI: https://doi.org/10.1590/S1415-43662010000400015

Schweinberger, C. M.; Putti, T. R.; Susin, G. B.; Trierweiler, J. O.; Trierweiler, L. F. (2016). Ethanol production from sweet potato: The effect of ripening, comparison of two heating methods, and cost analysis. The Canadian Journal of Chemical Engineering, v. 94, n. 4, p.716-724. https://doi.org/10.1002/cjce.22441 DOI: https://doi.org/10.1002/cjce.22441

Silva, L. A. de F.; Carvalho, S. A.; Florêncio Filho, D.; Alves, M. F.; Silva, N. L. T.; Gomes, J. R.; Santos, K. S. (2017). Produção de amilase por fungo filamentoso endofítico em fermentação submersa. Caderno de Ciências Agrárias, v. 9, p. 49-53.

Silva, L. F. L.; Gonçalves, W. M.; Maluf, W. R.; Resende, L. V.; Lasmar, A.; Carvalho, R. de C. (2019). Energy and budget balances for sweet potato-based ethanol production. Pesquisa Agropecuária Brasileira. https://doi.org/10.1590/S1678-3921.pab2019.v54.26521 DOI: https://doi.org/10.1590/s1678-3921.pab2019.v54.26521

Soares, I. A.; Flores, A. C.; Zanettin, L.; Pin, H. K.; Mendonça, M. M.; Barcelos, R. P.; Trevisol, L. R.; Carvalho, R. D.; Schauren, D.; Rocha, C. L. M. S. C.; Baroni, S. (2010). Identificação do potencial amilolítico de linhagens mutantes do fungo filamentoso Aspergillus nidulans. Ciência e Tecnologia de Alimentos, v. 30(3), p. 700-705. https://doi.org/10.1590/S0101-20612010000300021 DOI: https://doi.org/10.1590/S0101-20612010000300021

Teodoro, T. S.; Oliveira, F.; Poffo, C.; Braga, L. P.; Arbigaus, A.; Rampinelli, J. R.; Wisbeck, E.; Bonatti-Chaves, M.; Furlan, S. A. (2018). The influence of Tween 80 on laccase production by Pleurotus sajor-caju and the efficiency of crude enzyme broth in the removal of bisphenol-A. Arq. Inst. Biol., v.85, 1-10, e1022017. https://doi.org/10.1590/1808-1657001022017 DOI: https://doi.org/10.1590/1808-1657001022017

Tortora, G. J. (2012). Microbiologia, 10ª ed, Porto Alegre, Artmed. 89p.

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Publicado

05-12-2023

Como Citar

Mello Grizafis, V. K., Alberti Gomes, J., Alvim Veiga Pileggi, S., Masetto Antunes, S. R., Maidl de Souza, N., & Weirich Neto, P. H. (2023). Amylase potential of filamentous fungi isolated from sweet potato pulp. Revista Brasileira De Engenharia De Biossistemas, 17. https://doi.org/10.18011/bioeng.2023.v17.1126

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Regular Section