SEED QUANTITY ON IN VITRO CULTIVATION OF Dendrobium nobile LINDL. IN ALTERNATIVE CULTURE MEDIA WITHOUT SUBCULTURE
DOI:
https://doi.org/10.18011/bioeng2017v11n4p365-372Keywords:
Inoculation, micropropagation, OrchidaceaeAbstract
The in vitro orchid cultivation results in high percentages of germination when compared to the natural germination of these species, because their seeds have few or any nutritional reserves. The objective of this study was to evaluate different amounts of seeds in the in vitro propagation of Dendrobium nobile Lindl. var. nobilius and var. comet king in alternative medium, without subcultures. 0.02 g of seeds of each plant were used and disinfested in 0.8% sodium hypochlorite solution for 15 minutes, being diluted in sterile water until 60 mL was reached. In the aseptic environment, in vitro seeding was carried out 2, 3 or 4 mL of each seed suspension were pipetted into 600 mL glass vials provided with a metal stopper and containing 80 mL of the alternative culture medium. The cultures were transferred to the growth room with controlled temperature and photoperiod (25 ± 2 ºC, 16 hours) remaining for six months under these conditions. The highest number of potentially viable seeds (426 mg-1) was observed in D. nobile var. nobilius, while D. nobile var. comet king showed 164 mg-1 potentially viable seeds. The nobilius variety showed higher results in the total number of plants and live plants, number of leaves, number and diameter of pseudobulbs and length of the largest root, characteristic of this variety, which is more vigorous. The inoculation of 2mL was more efficient for the micropropagation of the two plants, with less competition between the seedlings in the flask.
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ABRÃO, M. C. R.; JORGE, J.; PESCADOR, R.; DE MELO FERREIRA, W.; SUZUKI, R. M. Germinação de sementes e desenvolvimento in vitro de plântulas de Cattleya loddigesii Lindl.(Orchidaceae). Revista Brasileira de Biociências, v. 12 (3):141-147, 2014.
ALVES, A. P.; SAPATINI, J. R.; SOUZA-LEAL, T.; FERNANDES, F. S.; MORAES, C. P. Influência da poda radical e recipientes na aclimatização ex vitro de Cattleya loddigesii Lindl. (Orchidaceae). Revista Brasileira de Biociências, v. 11 (4): 414-418, 2013.
ASSIS, A. M.; CARVALHO, J. F. R. P.; FARIA, R. T. Cultivo de orquídeas. Londrina: Mecenas, 2010. 208p.
CAMPOS D, M. Orquídeas: manual prático de cultura.Rio de Janeiro: Expressão e Cultura, 2002. 143p.
CORRIE, S.; TANDON, P. Propagation of Cymbidium giganteum Wall. Through high frequency conversion of encapsulated protocorms under in vitro and in vivo conditions. Indian Journal of Experimental Biology, v. 31(1): 61-64, 1993.
FARIA, R. T.; ASSIS, A. M.; UNEMOTO, L. K.; CARVALHO, J. F. R. P.Produção de orquídeas em laboratório. Londrina: Mecenas, 2012. 124 p.
JORGE, J.; JURAS, M. C. R.; SUZUKI, R. M. Germinação e crescimento inicial in vitro de Cattleya warneri T. Moore (Orchidaceae). Revista Brasileira de Biociências, v. 13(3): 134-141, 2015.
JUNQUEIRA, A. H.; PEETZ, M. S. Análise conjuntural do comércio exterior da floricultura brasileira. Revista Brasileira de Horticultura Ornamental, v. 16(1): 79-81, 2010.
KUHN, B. C.; CLAUDINO, L. O.; KUHN, S. B.; GUTIERRE, M. A. M.; MANGOLIN, C. A.; MACHADO, M. F. P. S. Micropropagação de Cattleya forbesii Lindley (Orchidaceae) usando combinações de auxina e citocinina. Revista Pleiade, v. 14(8): 73-82, 2014.
LAM, Y.; NG, T. B.; YAO, R. M.; SHI, J.; XU, K.; SZE, S. C. W.; ZHANG, K. Y. Evaluation of chemical constituents and important mechanism of pharmacological biology in Dendrobium plants. Evidence-Based Complementary and Alternative Medicine, v. 2015(1): 01-25, 2015.
LORENZI, H.Plantas para jardim no Brasil: Herbáceas, arbustivas e trepadeiras.Nova Odessa: Instituto Plantarum, 2013. 1120p.
MELLO, A. C. F.; PANTOJA, S. C. S. Orquídeas no paisagismo da área urbana de Ipanema-RJ. Revista Eletrônica Novo Enfoque, v. 18 (18): 87-108, 2014.
ROSA, Y. B. C. J.; RAMOS, F. Z.; SOUZA, R. G.; SOARES, J. S.; ROSA JUNIOR, E. J.; HOFFMANN, N. T. K.; ROSA, D. B. C. J.; SORGATO, J. C. Influência da luminosidade no crescimento e floração de Dendrobium nobile Lindl. Revista Brasileira de Horticultura Ornamental, v. 20 (1): 79-86, 2014.
SILVA, J. A. T.; TSAVKELOVA, E. A.; NG, T. B.; PARTHIBHAN, S.; DOBRÁNSZKI, J.; CARDOSO, J. C.; RAO, M. V.; ZENG, S. Asymbiotic in vitro seed propagation of Dendrobium. Plant cell reports, v. 34(10): 1685-1706, 2015.
SOARES, J. D. R., RODRIGUES, F. A., DE ARAUJO, A. G., PASQUAL, M., DE ASSIS, F. A. Crescimento in vitro de orquídeas: quantidade de meio e número de explantes. Ceres, v. 55(1): 49-53, 2008.
SORGATO, J.C.; ROSA, Y. B. C. J.; SOARES, J. S.; LEMES, C. S. R.; SOUSA, G. G. D. Light in intermediate acclimatization of in vitro germinated seedlings of Dendrobium phalaenopsis Deang Suree. Ciência Rural, v. 45(2): 231-237, 2015b.
SORGATO, J. C.; SOARES, J. S.; PINTO, J. V. C.; ROSA, Y. B. C. J. Potencial germinativo de sementes e qualidade de keikis de Dendrobium nobile em diferentes fases do desenvolvimento dos frutos. Ciência Rural, v. 45(11): 1965-1971, 2015a.
STANCATO, G. C.; BEMELMANS, P. F.; VEGRO, C. L. R. Produção de mudas de orquídeas a partir de sementes in vitro e sua viabilidade econômica: estudo de caso. Revista Brasileira de Horticultura Ornamental, v.17(1): 25-33, 2001.
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