A USEFUL METHODOLOGY TO SELECT LIPASE-CATALYZED TRANSESTERIFICATION AIMING BIODIESEL APPLICATION

F. D. A. Facchini, A. C. Vici, M. G. Pereira, M. F. de Oliveira, A. C. F. Batista, A. T. Vieira, T. A. Silva, J. A. Jorge, M. L. T. M. Polizeli

Resumo


The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO4.7H2O, 0.015% KH2PO4, 0.05% NH4H2PO4. Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production.


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DOI: http://dx.doi.org/10.18011/bioeng2016v10n1p01-13

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BIOENG, UNESP, Tupã, SP, Brasil. e-ISSN: 2359-6724

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